Lactophenol cotton blue stain fungal element stain for. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. Slide culture technique and lactophenol cotton blue. Staining in 5% blue cotton in lactophenol for 20 min macedo, 1997. Jorvet j326b lactophenol cotton blue stain indications.
Joining our editorial boards article and journal metrics. Most fungi can be identified on routine lactophenol cotton blue. Not available to individuals or residential addresses. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. This is a compound stain formed by interaction of methylene blue and eosin. For substitution suggestions, please call us at 800. Lactic acid acts as a preservative and it preserves the structure of fungus. Lactophenol cotton blue c37h27n3na2o9s3 is one of several stains that. Preparation of lactophenol cotton blue lpcb slide mounts. It is used as a stain in histology, and stains collagen blue in tissue sections. Lactophenol picric acid s017 intended use lactophenol picric acid is used as fungal staining solution. Composition ingredients formula adjusted, standardized to suit performance parameters directions principle and interpretation fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. Stain fungal elements with thermo scientific remel lactophenol aniline blue. Add one or two drops of lactophenol cotton blue stain before alcohol gets off.
Lactophenol cotton blue, fungal stain, 15ml, by hardy diagnostics. Lactophenol cotton blue wet mount is simple and widelthe y. Safety data sheet bactidroptm lactophenol aniline blue. Bbl lactophenol cotton blue stain droppers are used in wet amounts for. Lactophenol anilin blue lactophenol cotton blue component classification concentration glycerol cas no. A comparison of methodologies for the staining and quantification of. Linder recommended lactophenol as a mounting fluid for examination and preservation of fungi. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a. Lactophenol cotton blue stain fungal element stain for mycology. Bactidroptm lactophenol aniline blue safety data sheet date of issue 27092007. Lactophenol cotton blue s016 intended use lactophenol cotton blue is used for staining solution for fungi. What safe alternatives are there to lactophenol cotton. Lactophenol cotton blue is used for staining solution for fungi. Hivaids and fungal infections world health organization.
Staining of fungal cells by using lactophenol cotton blue. The modified method ie maygrunwald giemsa mgg technique is. It is a wet mount preparation fungal samples are simply transferred onto a slide and viewed wet. License creative commons attribution license reuse. Product identifier product name lactophenol cotton blue product number pl. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Remel bactidrop lactophenol aniline blue is for staining fungal elements. Lactophenol blue solution for microscopy, for staining. Jorvet j326b lactophenol cotton blue stain for animal use. Some article used methylen blue stain but i dont know about the concentration that i should use.
Lactophenol cotton blue lpcb for the staining and microscopic identification of fungi. Specimen mounts for microscopic examination of moulds includes teased, mashed, and slide culture preparations. Lactophenol blue stain is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. This product is intended to be used primarily with pure cultures, although certain specimens. Page 47 safety data sheet according to whs regulations printing date 29. Lactophenol cotton blue stain medical laboratories. Here in this mounting method we use lactophenol cotton blue stain where lactophenol acts as a cleaning agent as phenol is fungicidal in nature so it kills the organism. Lactophenol blue solution is a mounting medium and staining agent used in the. Preparation of lactophenol cotton blue slide mounts. About europe pmc funders joining europe pmc governance roadmap outreach. This staining procedure differentiates bacteria into gram positive and negative based. Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours.
Lactophenol blue solution for microscopy, for staining molds. Laboratory manual for diagnosis of fungal opportunistic infections in hivaids patients. Short video of using a lactophenol cotton blue stain for identifying fungal elements on a glass slide. Request pdf on sep 1, 2014, vacharavel shamly and others. After cooling, the sections can be examined under a microscope without taking the slides out of the dishes. A traditional stain which must be handled with care. Z7 the hardy diagnostics manufacturing facility and quality management system comply with the fdas good manufacturing practices gmp and have achieved iso 485 certification as a medical device. Material safety data sheet section 1 product and company information product name. Cotton blue is an acid dye that stains the chitin present. Other information msds first issued 28022007 msds data revised 27092007 uses stain for fungal elements. The material is stained in one step with lactophenol blue solution. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. Place the coverslip on the drop avoiding air bubbles to be trapped. Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain.
Comparison of microscopic morphology of fungi using. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Bbl lactophenol cotton blue stain droppers are used in wet amounts for examination of fungi 50sp, 500ca. Lactophenol cotton blue stain is recommended for mounting and staining yeast and molds. Fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. Many widely used fungal root stains, including trypan blue, are known carcinogens 11. Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% koh on a. New staining technique for fungalinfected plant tissues.
Organisms suspended in the stain are killed due to the presence of phenol. Lactophenol aniline blue is recommended by koneman, roberts, and wright for direct mounting and staining of fungi. I read some of journal and they used lactophenol cotton blue stain but its hard to find it here. Lactophenol blue solution is readytouse staining solution for fungi staining in bacteriology with samples of human origin. Iodineglycerol as an alternative to lactophenol cotton blue for identification of fungal elements in clinical laboratory. The fungal structures include mycelium, sporangiospore, spores etc. Lactophenol cotton blue blue is convenient for day to day use. The slides were analysed, and the images were captured with a leica dc 300f digital camera attached to a leica dmld microscope.
Formalincongo redstained tease mount preparations of zygomyces. Bbltm lactophenol cotton blue stain droppers, catalog number 261188, shelf package of 50 droppers, incorrectly labeled in part methylene blue loefflerbecton dickinson and companypackaged in mexico lactophenol cotton blue is a blue stain used for direct examination of clinical specimens for fungal elements. Phenol 10 29% 108952 water 30 60% 7732185 lactic acid 10 29% 50215 glycerol 10 29% 56815 classified as hazardous according to nohsc criteria synonyms cotton blue, fronine lacto cotton blue. Methyl blue is a chemical compound with the molecular formula c 37 h 27 n 3 na 2 o 9 s 3. On exposure to acids, alkali and ultraviolet light, a number of oxidation products methylene azures are formed from methylene blue which give contrast staining. Lactophenol, staining solutions, by loba chemie, india. If the fungal hyphae are not sufficiently stained, take the slides back to cotton blue and heat.
Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Bd stain droppers lactophenol cotton blue life sciences. For selective study you can make use of other strain as given by. The aim of the lactophenol cotton blue lpcb technique is to provide a quick and easy method of staining fungi for observation under the light microscope. This item is sold and shipped to schools and businesses only. Check for and remove contact lenses if present and easy to do. It is known to be most commonly used for microscopic study of fungi. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue.
Comparison of microscopic morphology of fungi using lactophenol. Safety data sheet lactophenol cotton blue according to regulation ec no 19072006, annex ii, as amended by regulation eu no 4532010 section 1. Cool, drain off excess of staining fluid and place in smaller dishes of lactophenol diluted with methylated spirit. Staining of clinical specimens nonkeratinized procedure lcb staining 1. Place a drop of seventy percent alcohol on a clean microscope slide. Staining of fungus by using lactophenol cotton blue. An area of limitation within the method used arises from the manual counting of. A safer alternative to lactophenol for mycology is also available cotton blue in lactic acid. Chem alert report manufacturers material safety data sheet product name lacto cotton blue ingredient conc. Washable polyestercotton blend extends lifespan of chairs by preventing dirt.